Topic outline

  • Introduction in Instrumental Analysis.

    •  Introduction to instrumental analyses.
    •  Classification of methods.
    •  Advantages and disadvantages of instrumental methods over chemicals ones.

    • Statistical Analysis

      Analytical methods characteristics (reliability, selectivity, sensitivity). Quantification techniques for data analysis (standard curves, limits of detection (LOD), limits of quantification (LOQ), internal standard addition method). Precision and accuracy monitoring. Basic Statistical Analysis Methods

      • Basics of Spectroscopy

        • Introduction to spectroscopic methods
        • Components of optical systems

        • Atomic Spectroscopy

          • An introduction to specrtometric methods.
          • Components of optical instruments.
          • Atomic absorption spectroscopy.
          • Atomic emission spectroscopy

          • Molecular Spectroscopy – Electronic transitions

            • Introduction to UV-Vis molecular spectroscopy
            •  Applications of UV-Vis spectroscopy  
            •  Fluorescence, phosphorescence and chemiluminescence

            • Molecular Spectroscopy – Vibrational excitation

              • IR absorption spectroscopy
              1. Requirements for infrared absorption
              2. Energy levels in vibrating and rotating molecules
              3. Equipment: IR and FT IR spectrometer (radiation source, monochromators, detectors, sample cells)
              4. Fourier transformation detector systems
              5. Applications of Infrared Spectrometry

              • Additional Instrumental Methods for Organic Structural Analysis

                • Mass Spectrometry

                • Electroanalytical Chemistry

                  • Introduction to electroanalytical  chemistry
                  • Potenciometry, pH and ion-selective electrodes
                  • Coulometry

                  • Separation Methods

                    • An introduction to chromatographic separation
                    1. Van Deemter equation,
                    2. Theoretical plate concept
                    • Gas chromatography (GC), theory, and equipment.
                    1. Different detectors and columns used in GC and their application.
                    2. Demonstration of capillary and packed columns
                    3. Demonstration of flame ionization (FID) and electron capture (ECD) detectors
                    • High – performance liquid chromatography (HPLC)
                    1. detectors,
                    2.  normal phase versus reversed phase chromatography, ion exchange, gel permeation

                    • Electrophoresis

                      • Theory of electrophoresis, methods of electrophoresis, PAGE, IF, CE.
                      • Polyacrylamide Gel Electrophoresis (PAGE) of Proteins
                      • Demonstration of capillary electrophoresis equipment (BioRad - BioFocus 3000), experimental setup, method development, various applications.